Metabolomics with the Q Exactive
Nov
02,
2020
-
Nov
04,
2020
Overview
This 3-day course introduces you to using the Q Exactive mass spectrometer in your metabolomics investigations. The course is led by experts in the field of metabolomics and includes lectures, laboratory sessions and computer workshops to provide a detailed overview of the metabolomics pipeline applying the Q Exactive mass spectrometer.
Topics covered
- Introduction to Metabolomics on the Q Exactive, the metabolomics workflow, and case studies using the Q Exactive
- Using the Q Exactive family of instruments in your metabolomics investigations
- Experimental design and the importance of quality control samples
- Sample preparation including polar and non-polar preparation methods on biofluids (urine and plasma) and tissue samples
- Preparation of samples for profiling and targeted analyses on the Q Exactive
- Hands-on data acquisition for profiling and targeted studies, setting up the Vanquish UHPLC coupled to the Q Exactive MS
- Data processing workshop
- Data analysis workshop (univariate and multivariate analysis)
- Introduction to metabolite identification applying Data Dependent Analysis and Data Independent Analysis
- Question and answer session with a panel of experts
- Tips and tricks
- Problem solving