Overview
This 2-day course will provide a hands-on approach to teach the attendees about the latest techniques and tools available to perform metabolite identification in non- targeted metabolomics studies. The course will be led by experts working within the fields of metabolomics and chemical analysis and will include a significant proportion of hands-on experience of using mass spectrometers, software tools and databases. A maximum of four people will be working on each mass spectrometer in a session. We will apply these tools on the Q Exactive and LTQ-Orbitrap family of mass spectrometers.

Topics covered

• Importance of mass spectral interpretation
• Types of data which can be collected on the QE and LTQ-Orbitrap (m/z, retention time, MS/MS, MSn)
• Conversion of raw data to molecular formula and putative metabolite annotations
• MS/MS experiments in metabolic phenotyping for on-line data acquisition using the QE (DDA, DIA, all- ion)
• MS/MS and MSn experiments for sample fractions using the LTQ-Orbitrap
• Mass spectral libraries (using mzCloud)
• Searching mass spectral libraries
• Tools for mass spectral interpretation
• Reporting standards for metabolite identification
• Question and answer session with the experts

Course link: https://www.birmingham.ac.uk/facilities/metabolomics-training- centre/courses/2020/Metabolite-identification-with-the-Q-Exactive-and-LTQ-Orbitrap-Elite-November- 2020.aspx