Course Topics:

• Introduction to QE+ and ID-X mass spectrometers and the metabolomics workflow
• Experimental design and the importance of quality control (QC) samples
• Sample preparation including extraction of polar metabolites and lipids from biofluids (urine and plasma) and tissue samples
• Hands-on data acquisition for untargeted metabolomics and hybrid metabolomics, the latter combining targeted and untargeted measurements
• Setting up the Vanquish UHPLC coupled to the QE+
• Types of data which can be collected on the QE+ and ID-X (m/z, retention time, MS/MS, MSn)
• MS/MS and MSn experiments
• Data processing workshop
• Data analysis workshop (univariate and multivariate analysis)
• Introduction to metabolite identification applying Data Dependent Analysis
• Conversion of raw data to molecular formula and putative metabolite annotations
• Mass spectral libraries (using mzCloud), and how to search them
• Tools for mass spectral interpretation
• Reporting standards for metabolite identification

Question and answer session with a panel of experts

Event website:  https://www.birmingham.ac.uk/facilities/metabolomics-training-centre/courses/2026/metabolomics-lc-ms-data-processing-and-statistical-analysis