This course provided an introduction to metabolomics through lectures and hands-on sessions, using publicly available data, software, and tools. Participants became familiar with the current state of experimental design, data acquisition (LC-MS, MS imaging), processing, and modelling. In addition, they learned about community standards and sharing in metabolomics, particularly through using the EMBL-EBI’s MetaboLights repository and Galaxy infrastructure. Participants learned through hands-on tutorials to use tools available for data analysis and data submission. Additionally, case studies were used to discuss and show how to employ the week’s learning. For the full program information, view the course website.